Molecular Genetics of Prokaryotes Problem Set

Problem 4: Exchange of genetic information between bacteria

Cells of an E. coli strain that are trp lac Z met bio were mixed with cells of an E. coli strain that are trp lac Z met bio and cultured for several hours. Then cells were removed, washed, and transferred to minimal media containing lactose as the only sugar source. A few cells were able to grow on minimal media with lactose, and formed colonies. How did these few cells become trp lac Z met bio?

A. transformation
B. transduction
C. sexduction
D. conjugation
Genetic conjugation between bacteria was first described by Lederberg and Tatum in 1946.
E. transposition


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