Molecular Genetics of Prokaryotes Problem Set
Problem 5: DNA transfer by an F factor
An E. coli strain is F lac Z met bio . Cells from this strain are mixed with an E. coli strain that is lac Z met bio and carrying an F' episome with the plac O lac Z DNA sequence on the episome, and cultured for several hours. Then cells were removed, washed, and transferred to minimal media containing lactose as the only sugar source. A few cells were able to grow on minimal media with lactose, and formed colonies. How did these few cells become
lac Z met bio ?
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Updated: July 15, 1999
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