ELISA Activity
Problem 3
What would happen if the anti-human Ig-conjugate were not washed free of the well before the substrate was added?
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The ELISA would not develop when the substrate was added. |
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The ELISA would develop normally. |
![](graphics/c.gif) |
All wells would show uniform over development due to unbound and excess anti-human Ig enzyme conjugate. |
![](graphics/d.gif) |
Both A and B. |
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The Biology Project
The University of Arizona
Wednesday, January 22, 1998
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