ELISA Activity
Problem 2
What would happen if serum were omitted from the ELISA, but all other steps remained the same and were performed properly?
Anti-human Ig-conjugate would not bind and be washed away. Anti-human Ig-conjugate would bind non-specifically to the ELISA plate. The O.D. values would be nearly the same as the assay control. Both A and C.
The University of Arizona
Wednesday, January 22, 1998
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