ELISA Activity

Question 3

What would happen if the anti-human Ig-conjugate were not washed free of the well before the substrate was added?

The ELISA would not develop when the substrate was added.

Incorrect.
The substrate would overdevelop because an excess of enzyme coupled to the anti-human Ig was present.

The Biology Project
University of Arizona
Monday, March 31, 1997
denicew@u.arizona.edu