ELISA Activity

Question 2

What would happen if serum were omitted from the ELISA, but all other steps remained the same and were performed properly?

Anti-human Ig-conjugate would bind non-specifically to the ELISA plate.

Incorrect.The anti-human Ig conjugate is specific for human Ig and has very little nonspecific binding activity.

The Biology Project
University of Arizona
Monday, March 31, 1997
denicew@u.arizona.edu