## Blackett Family DNA Activity 2

### DNA Profile Frequency Calculations

In this activity, you can calculate the probability for some of the DNA profiles you have been studying. The following set of data are tables of allele frequency for the three STR loci D3S1358, vWA, and FGA for a combined, Caucasian population. The frequency data come from the web site of the Royal Canadian Mounted Police.

 Locus Allele Frequency D3S1358 12 0.015 D3S1358 13 0.015 D3S1358 14 0.1341 D3S1358 15 0.2896 D3S1358 16 0.2287 D3S1358 17 0.1616 D3S1358 18 0.1616 D3S1358 19 0.0152

 Locus Allele Frequency VWA 12 0.015 VWA 14 0.1311 VWA 15 0.1189 VWA 16 0.186 VWA 17 0.2774 VWA 18 0.189 VWA 19 0.0884 VWA 20 0.015

 Locus Allele Frequency FGA 18 0.015 FGA 19 0.061 FGA 20 0.125 FGA 21 0.1799 FGA 22 0.2287 FGA 23 0.1311 FGA 24 0.1463 FGA 25 0.0945 FGA 26 0.0183 FGA 27 0.015

1. What is the Probability for a 3-Locus DNA profile?
Based on a population database of Caucasians developed by Bob Blackett and colleagues in Arizona, Bob can calculate the genotype frequency of his combined profile for the three STR loci D3S1358, vWA, and FGA to be 6 x 10-5. Compare this frequency with the frequency you calculate from the Royal Canadian Mounted Police data. For help with this calculation, review the DNA Profile Frequency Calculation page.
2. Genotype Frequency for the 13 CODIS STR Loci.
If you feel really ambitious, retrieve additional frequency data for the other 10 STR Loci from the web site of the Royal Canadian Mounted Police and calculate the genotype frequency for all 13 STR loci for one or more individuals from this study.
As an alternative to doing all of the arithmetic yourself, you can Calculate a profile's Random Match Probability using the RCMP on-line calculator.

Introduction : Overview | STR P | CODIS | Analysis | Inheritance | Frequency Calc.

Activities : Pedigree | Collect data | Paternity testing | Missing person | RCMP freq. calc.

The Biology Project
University of Arizona
October 27, 2000
hallick@email.arizona.edu
http://biology.arizona.edu