Nucleic Acids and the Genetic Material Problem Set 1

Problem 2: 14N distribution after one generation

In the Meselson-Stahl DNA replication experiment, what percent of the DNA was composed of one light strand and one heavy strand after one generation of growth in 14N containing growth media?
A 0

B 25

C 50

D 75

E 100

During semiconservative replication, each of the dense strands of parental DNA serves as a template for the synthesis of new DNA. After one generation, all of the daughter DNA's will include one single strand of parental DNA (heavy) and one single strand of newly synthesized DNA (light).

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Thursday, October 3, 1996
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