Recombinant DNA Technology Problem Set
Problem 5 Recombinant DNA 3
Correct!
Which of the following is not part of the normal process of cloning recombinant
DNA in bacteria?
A.
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restriction endonuclease digestion of cellular and plasmid DNAs.
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B.
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production of recombinant DNA using DNA ligase and a mixture of digested cellular and
plasmid DNAs.
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C.
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separation of recombinant DNAs by electrophoresis using the Southern technique to
determine where the desired recombinant migrates.
Yes, the Southern technique is used to detect where fragments of DNA migrate on a gel during electrophoresis.
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D.
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transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin.
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E.
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probing blots of bacteria clones with radioactive DNA complementary to the desired
gene.
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The Biology Project
University of Arizona
Tuesday, October 29, 1996
Contact the Development Team
http://biology.arizona.edu
All contents copyright © 1996. All rights reserved.
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