Recombinant DNA Technology Problem Set
Problem 5 Recombinant DNA 3
Which of the following is not part of the normal process of cloning recombinant
DNA in bacteria?
A.
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restriction endonuclease digestion of cellular and plasmid DNAs.
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B.
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production of recombinant DNA using DNA ligase and a mixture of digested cellular and
plasmid DNAs.
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C.
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separation of recombinant DNAs by electrophoresis using the Southern technique to
determine where the desired recombinant migrates.
The Southern technique is used to detect where fragments of DNA migrate on a gel during electrophoresis.
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D.
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transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin.
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E.
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probing blots of bacteria clones with radioactive DNA complementary to the desired
gene.
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The Biology Project
University of Arizona
Tuesday, October 29, 1996
Contact the Development Team
http://biology.arizona.edu
All contents copyright © 1996. All rights reserved.
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