ELISA Activity

ELISA data from three patients

Positive Control Negative Control Patient A Patient B Patient C Assay Control
1.689 0.153 O.055 0.412 1.999 0.123

Above is ELISA data from three patients. Numbers are expressed as optical density at 450 nm. The cutoff value indicating a positive result is 0.500. Optical densities of 0.300 to 0.499 are indeterminate and need to be retested. Values below 0.300 are considered to be negative. In most cases, a patient will be retested if the serum gives a positive result. If the ELISA retests are positive, the patient will then be retested by western blotting analysis.

The Biology Project
The University of Arizona
Thursday, January 21, 1998
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