list of lesson plans
The Arizona Hedgehog
Endangered Species or not?
- List the morphological characters which are used to identify the
cacti in the field, and be able to describe the associated problems
in using these characters for identification.
- Draw the geographical ranges of Echinocereus triglochidiatus var.
arizonicus and related cactus populations.
- Formulate a hypothesis regarding the validity of the current status
of the Arizona Hedgehog cactus as a distinct variety of cactus,
taking into consideration the amount of diversity found within each
population and between the two populations in question.
- List and describe the steps taken to isolate DNA from plant tissue,
explaining the chemical and physical properties used.
- List and describe the three steps used in Polymerase Chain Reaction
and explain how repeating these three steps amplifies DNA exponentially.
- Explain how amplification of DNA with a "random", nonspecific
primer can provide a "DNA fingerprint" for comparing similarities
and differences among individuals and populations.
- Describe how DNA moves through an agarose gel and how fragments
of DNA are separated by size.
- Explain how different banding patterns between individuals relate
to differences in DNA sequence.
- Explain and perform how an agarose gel is poured and DNA is loaded
into the wells of the gel.
- Analyze banding patterns for similarities and differences among
individuals and between populations.
- Describe and explain the content of each section in the format
of a scientific paper or poster: title, author, abstract, introduction,
materials and methods, results, discussion and bibliographic references.
- Draw a conclusion about the classification of the Cochise county
cacti and make recommendations about the need for protective efforts
for the Arizona Hedgehog cactus (arizonicus).