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The Arizona Hedgehog Cactus: Endangered Species or not?

By Deborah Alongi Johnson

Master Materials List

Lesson 1:

  • Class Internet access or single computer with projection screen
  • Slides of the Arizona Hedgehog and related varieties
  • Student copies of "Investigation of the Problem".
  • Student copies of "Classification of Echinocereus triglochidiatus and Geographical Ranges of its Varieties".

Lesson 2:

  • Student copies of "Chemical Detectives: How to Find and Isolate DNA"
  • Student copies of "DNA Isolation from Plant Tissue"

Lesson 3:

  • Plant tissue such as pea or Desert Spoon
  • Coffee grinders
  • Dry ice
  • 50-ml conical polypropylene or glass tubes
  • Centrifuge
  • Hot water bath at 60°C
  • CTAB buffer (available from research lab)
  • Chloroform
  • Fume hood (or well-ventilated area)
  • Pipettes
  • Ice-cold isopropanol
  • Student copies of "Isolation of DNA from Plant Tissue"

Lesson 4:

  • Overhead transparency diagrams of PCR and amplification with single- sequence 10-bp primers
  • Student copies of background reading "The Polymerase Chain Reaction (PCR) "
  • Student copies of "Polymerase Chain Reaction Amplification of DNA"

Lesson 5:

  • Campus playing field, gym or large classroom
  • Old tires, cones, desks, chairs, etc.
  • Candy (reward)
  • Overhead transparency of "A DNA Agarose Gel"
  • Student copies of "Gel Electrophoresis and Analysis"

Lesson 6:

  • 100X electrophoresis buffer stock
  • Deionized water
  • 100 ml and 1000 ml graduated cylinders
  • Methylene blue dye or ethidium bromide
  • Centrigram balance
  • Agarose
  • Large test tubes
  • Gel casting trays with combs (and glass plates if necessary)
  • Masking tape
  • Plastic pipets
  • Boiling water bath
    •

Lesson 7:

  • Same as Lesson 6
    •

Lesson 8:

  • Light box for viewing gels
  • Polaroid camera and film

Lesson 9:

  • Student copies of "Publication of Procedure and Results"
  • Student copies of "Evaluating the Relationship of Autumn Buttercup to Some Close Congeners Using Random Amplified Polymorphic DNA"
The University of Arizona
 Department of Biochemistry and Molecular Biophysics
 General Biology Program for Secondary Teachers
warder@email.arizona.edu

http://biology.arizona.edu/sciconn/lessons2/lessons.html
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