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Enables a scientist to make many copies of
a target region from an original double-stranded DNA molecule
(called the template DNA)
Step 1: Separation of the DNA strands by heating to 95°C.
Step 2: Bind single-stranded primers to complementary sequences on both separated template DNA strands (55°C).
Step 3: Extend primers with Taq polymerase, which brings in new nucleotides to build new DNA strands (72°C).
| Science Education Connection
Department of Biochemistry The University of Arizona May 1, 1997 warder@u.arizona.edu
http://biology.arizona.edu/sciconn/lessons/alongi/ |